甘油三酯酶法测定试剂盒

甘油三酯由甘油与脂肪酸酯化而成，是血脂的主要成分，也是机体重要的供能物质。高甘油三酯是临床常见的高脂血症，与多种疾病有关。除了血浆甘油三酯外，组织、细胞中的甘油三酯聚集增加，也与肥胖、脂肪肝、胰岛素抵抗、能量代谢异常等诸多病理过程密切相关。  实体组织和细胞的甘油三酯测定远比与血液甘油三酯测定复杂。试剂盒避免了有毒的氯仿甲醇提取、繁杂的氮气吹干和脂质复溶等步骤，采用高效能试剂进行裂解和甘油三酯抽提，以及优化的GPO Trinder酶学反应组分和操作步骤。简单易行，灵敏度高，线性范围20~2000 µmol/L。用于精确测量动物和植物的组织、细胞、以及固态食品材料、含高浓度油料样品中的甘油三酯。

使用甘油三酯酶法测定试剂盒（E1013）发表的部分论文：
1、Zhang Xuequn, Yang Juntao, Guo Yuanbiao, Ye Hua, Yu Chaohui, Xu Chengfu, Xu Lei, Wu Songfeng, Sun Wei, Wei Hangdong, Gao Xue, Zhu Yunping, Qian Xiaohong, Jiang Ying, Li Youming, He Fuchu, 2009. Functional proteomic analysis of nonalcoholic fatty liver disease in rat models: Enoyl–coenzyme a hydratase down-regulation exacerbates hepatic steatosis. Hepatology, 51(4): 1190-1199. 

2、Luo Zhidan, Ma Liqun, Zhao Zhigang, He Hongbo, Yang Dachun, Feng Xiaoli, Ma Shuangtao, Chen Xiaoping, Zhu Tianqi, Cao Tingbing, Liu Daoyan, Nilius Bernd, Huang Yu, Yan Zhencheng, Zhu Zhiming, 2011. TRPV1 activation improves exercise endurance and energy metabolism through PGC-1α upregulation in mice. Cell Research, doi:10.1038/cr.2011.205. 

3、Pang Shanshan, Tang Haiqing, Zhuo Shu, Qin Zang Ying, Le Yingying, 2010. Regulation of Fasting Fuel Metabolism by Toll-Like Receptor 4. Diabetes, 59(12): 3041-3048. 

4、Ding Yunfeng, Yang Li, Zhang Shuyan, Wang Yang, Du Yalan, Pu Jing, Peng Gong, Chen Yong, Zhang Huina, Yu Jinhai, Hang Haiying, Wu Peng, Yang Fuquan, Yang Hongyuan, Steinbuchel Alexander, Liu Pingsheng, 2011. Identification of the major functional proteins of Prokaryotic lipid droplets. The Journal of Lipid Research, doi:10.1194/jlr.M021899.

5、Zhao Zhigang, Luo Zhidan, Wang Peijian, Sun Jing, Yu Hao, Cao Tingbing, Ni Yinxing, Chen Jing, Yan Zhencheng, Liu Daoyan, Zhu Zhiming, 2011. Rosiglitazone Restores Endothelial Dysfunction in a Rat Model of Metabolic Syndrome through PPARγ- and PPARδ-Dependent Phosphorylation of Akt and eNOS. PPAR Research, doi:10.1155/2011/291656.

6、Deng Jiagang, Li Chunyang, Wang Hailian, Hao Erwei, Du Zhengcai, Bao Chuanhong, Lv Jianzhen, Wang Yi, 2011. Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells. Biochemical and Biophysical Research Communications, 411(3): 523–529.

7、Zhai, W., Xu, C., Ling, Y., Liu, S., Deng, J., Qi, Y., Londos, C., Xu, G., 2010. Increased Lipolysis in Adipose Tissues is Associated with Elevation of Systemic Free Fatty Acids and Insulin Resistance in Perilipin Null Mice, Hormone Metabolic Research, 42: 247-253.

8、Yang Zhi, Yin Ji-Ye, Gong Zhi-Cheng, Huang Qiong, Chen Hao, Zhang Wei, Zhou Hong-Hao, Liu Zhao-Qian, 2009. Evidence for an effect of clozapine on the regulation of fat-cell derived factors. Clinica Chimica Acta, 408(1–2): 98-104.

9、He Yong-Han, He Ying, Liao Xi-Lu, Niu Yu-Cun, Wang Guan, Zhao Chen, Wang Liang, Tian Ming-Jie, Li Ying, Sun Chang-Hao, 2012. The calcium-sensing receptor promotes adipocyte differentiation and adipogenesis through PPARγ pathway. Molecular and Cellular Biochemistry, 361(1-2): 321-328. 

10、Ju Dapeng, He Jingjing, Zhao Lili, Zheng Xueli, Yang Gongshe, 2011. Estrogen related receptor α-induced adipogenesis is PGC-1β-dependent. Molecular Biology Reports, 39(3): 3343-3354, DOI: 10.1007/s11033-011-1104-8.