膜再生液

膜再生液（P1650）允许对同一张膜进行多次Western Blot检测。在室温条件下，将用过的膜浸泡在膜再生液中30分钟，能够选择性清除与膜抗原结合的第一抗体及第二抗体，但不影响电转移到膜上的蛋白。随后，可以使用不同的抗体进行下一轮Western Blot实验，因而可利用同一张膜进行多次蛋白检测。不仅适宜用少量样品多次检测多种不同蛋白，即使样品量并不匮乏，采用这种多次检测同一张膜上的蛋白的策略，能省去给药处理、蛋白电泳和膜转移等耗费性步骤。

特点： （1）无毒无味无害，室温保存和使用；

            （2）可对同一张膜进行10次以上的Western Blot检测。

部分使用普利莱膜再生液发表的SCI文章，供参考：

1、 Zhao X, Gao M, He J, et al. Perilipin1 Deficiency in Whole Body or Bone Marrow-Derived Cells Attenuates Lesions in Atherosclerosis-Prone Mice[J]. PloS one, 2015, 10(4).

2、 Deng J, Liu S, Zou L, et al. Lipolysis response to endoplasmic reticulum stress in adipose cells[J]. Journal of Biological Chemistry, 2012, 287(9): 6240-6249.

3、 Wang J Y, Xia Q, Chu K T, et al. Severe global cerebral ischemia-induced programmed necrosis of hippocampal CA1 neurons in rat is prevented by 3-methyladenine: a widely used inhibitor of autophagy[J]. Journal of Neuropathology & Experimental Neurology, 2011, 70(4): 314-322.

4、 Jia C, Zhu W, Ren S, et al. Comparison of genome-wide gene expression in suture-and alkali burn-induced murine corneal neovascularization[J]. Molecular vision, 2011, 17: 2386.

5、  Huang M, Liu X, Du Q, et al. Inhibitory effects of sunitinib on ovalbumin-induced chronic experimental asthma in mice[J]. Chin Med J (Engl), 2009, 122(9): 1061-1066.

6、 Zhang T, He J, Xu C, et al. Mechanisms of metformin inhibiting lipolytic response to isoproterenol in primary rat adipocytes[J]. Journal of molecular endocrinology, 2009, 42(1): 57-66.

7、Zhang G R, Cheng X R, Zhou W X, et al. Age-related expression of calcium/calmodulin-dependent protein kinase II A in the hippocampus and cerebral cortex of senescence accelerated mouse prone/8 mice is modulated by anti–Alzheimer's disease drugs[J]. Neuroscience, 2009, 159(1): 308-315.