胞核-胞浆-胞膜制备试剂盒
试剂盒能够从哺乳动物新鲜或冻存的组织块、贴壁或悬浮细胞中制备细胞核、细胞膜与胞内质膜、细胞浆等三种主要亚细胞组分。其独特的试剂成分与优化的制备方案使胞核-胞浆-胞膜制备过程简单易行,无需特殊设备和超速离心,可在1小时内完成。应用本试剂盒制备的胞膜是细胞膜和细胞器膜如线粒体、内质网及质膜的混合物,得到的细胞核是完整的未裂解细胞核,胞浆组分为可溶性胞浆蛋白。制备得到的产物纯度可胜任后续的免疫沉淀、蛋白印迹、2-D gel、酶活性检测和受体分析等实验。
数篇使用普利莱P1201试剂盒发表的文章供参考。
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