微信登录
收藏本站
蛋白酶抑制剂混合物通用型 (进口原料Cocktail,50x) P1265厂家直销，提供OEM定制服务，大包装更优惠
- 产品详情
- 说明书下载
- 引用文献
描述：当细胞破碎的时候，蛋白水解酶就会被释放出来或被激活，使电泳结果复杂化，影响最终结果分析。为了避免这些情况的出现，建议在样品制备时尽可能在低温操作。此外，许多蛋白酶在pH9以上会失活，因此Tris碱、碳酸钠、碱性载体、两性电解质等往往能抑制蛋白水解，但是有些蛋白酶在上述条件下仍然保持着活性，此时应当使用蛋白酶抑制剂。因此对于我们想要研究的蛋白来说，选择性和有效地抑制相应蛋白酶活性对于蛋白纯化路线设计来说显得尤为重要。
规格：
1mL 2mL
储存：
-20℃一年
操作步骤：
1. 取出蛋白酶抑制剂混合物(Protease inhibitor cocktail, 50x)，振荡重悬。
2. 按照1:49比例，将混合物加入组织细胞裂解液中，混合均匀即可。
1. Qiu L, Yue L, Ni Y, et al. Emodin-induced oxidative inhibition of mitochondrial function assists BiP/IRE1α/CHOP signaling-mediated ER-related apoptosis[J]. Oxidative Medicine and Cellular Longevity, 2021, 2021.
2. Zou S, Wei Z Z, Yue Y, et al. Desflurane and surgery exposure during pregnancy decrease synaptic integrity and induce functional deficits in juvenile offspring mice[J]. Neurochemical Research, 2020, 45: 418-427.
3. Chang S, Hu L, Xu Y, et al. Inorganic nitrate alleviates total body irradiation-induced systemic damage by decreasing reactive oxygen species levels[J]. International Journal of Radiation Oncology* Biology* Physics, 2019, 103(4): 945-957.
4. Zhang M, Xie Z, Gao W, et al. Quercetin regulates hepatic cholesterol metabolism by promoting cholesterol-to-bile acid conversion and cholesterol efflux in rats[J]. Nutrition research, 2016, 36(3): 271-279.
5. Zhang Z, Liu H, Zhao Z, et al. GJ-4 alleviates Aβ25-35-induced memory dysfunction in mice through protecting the neurovascular unit[J]. Biomedicine & Pharmacotherapy, 2020, 127: 110131.
6. Gong X, Yu Y, Tong Q, et al. Effects of “Bu Shen HuoXue Decoction” on the endometrial morphology and expression of leukaemia inhibitory factor in the rat uterus during the oestrous cycle[J]. Evidence-based Complementary and Alternative Medicine, 2013, 2013.
7. Weng J, Han X, Liu K, et al. CD44 3'-untranslated region functions as a competing endogenous RNA to enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression[J]. International journal of biological sciences, 2019, 15(8): 1664.
8. Gong X, Lou J, Lu Q, et al. Bu Shen HuoXue decoction restores endometrial leukemia-inhibitory factor but not Angiopoietin-2 expression, and improves uterine receptivity in the controlled ovarian stimulation rat model[J]. Experimental and Therapeutic Medicine, 2015, 9(3): 751-757
9. Liu Q, Liu S, Gao L, et al. Anti-diabetic effects and mechanisms of action of a Chinese herbal medicine preparation JQ-R in vitro and in diabetic KKAy mice[J]. Acta pharmaceuticasinica B, 2017, 7(4): 461-469
10. Bu X, Liu Y, Lu Q, et al. Effects of “danzhi decoction” on chronic pelvic pain, hemodynamics, and proinflammatory factors in the murine model of sequelae of pelvic inflammatory disease[J]. Evidence-Based Complementary and Alternative Medicine, 2015, 2015.
11. Ding C, Li Y, Wang S, et al. ROBO2 hampers malignant biological behavior and predicts a better prognosis in pancreatic adenocarcinoma[J]. Scandinavian Journal of Gastroenterology, 2021, 56(8): 955-964
12. Shao M, Wang M, Ma L, et al. β-elemene blocks lipid-induced inflammatory pathways via PPARβ activation in heart failure[J]. European Journal of Pharmacology, 2021, 910: 174450.
13. Zhang J, Lin W, Tang M, et al. Inhibition of JNK ameliorates depressive-like behaviors and reduces the activation of pro-inflammatory cytokines and the phosphorylation of glucocorticoid receptors at serine 246 induced by neuroinflammation[J]. Psychoneuroendocrinology, 2020, 113: 104580.
14. Liu X, Jia R, Meng X, et al. Retinal degeneration in humanized mice expressing mutant rhodopsin under the control of the endogenous murine promoter[J]. Experimental Eye Research, 2022, 215: 108893.
15. Wu C, Zheng W, Jia X, et al. Adolescent chronic unpredictable stress causes a bias in goal‐directed behavior and distinctively changes the expression of NMDA and dopamine receptors in the dorsomedial and dorsolateral striatum in male rats[J]. Developmental Psychobiology, 2022, 64(2): e22235.
16. Jiang M, Wang W, Zhang J, et al. Protective effects and possible mechanisms of actions of BushenCuyun recipe on diminished ovarian reserve induced by cyclophosphamide in rats[J]. Frontiers in Pharmacology, 2020, 11: 546.
17. Qiu L Z, Zhou W, Yue L X, et al. Repeated Aconitine Treatment Induced the Remodeling of Mitochondrial Function via AMPK–OPA1–ATP5A1 Pathway[J]. Frontiers in Pharmacology, 2021, 12: 646121.
18. Shen Y, Li M, Xiong Y, et al. Proteomics analysis identified ASNS as a novel biomarker for predicting recurrence of skull base chordoma[J]. Frontiers in oncology, 2021, 11: 698497.
19. Zhang Y, Shen Y, Li M, et al. Loss of INI1 inhibits the expression of SIDT1 and promotes tumor progression in skull base chordoma by regulating EZH2-mediated H3K27me3[J]. 2022.
20. Liu S, Liu Q, Li L, et al. Long-term fenofibrate treatment impaired glucose-stimulated insulin secretion and up-regulated pancreatic NF-kappa B and iNOS expression in monosodium glutamate-induced obese rats: is that a latent disadvantage?[J]. Journal of translational medicine, 2011, 9: 1-11.
21. Tang X, Li J, Zhao W, et al. Comprehensive map and functional annotation of the mouse white adipose tissue proteome[J]. PeerJ, 2019, 7: e7352.
22. Liu Q, Shen Y, Xiong Y, et al. Comparative Efficacy of AZD9496 and Fulvestrant on the Growth of Pituitary Adenoma via Blocking JAK2/STAT5B Pathway[J]. Journal of Cancer, 2023, 14(1): 61-71.
23. Wang Y, Wang Q, Guo C, et al. Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects[J]. Molecular Medicine Reports, 2015, 12(2): 3089-3094.
24. Zhao Z, Ning J, Bao X, et al. Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis[J]. Microbiome, 2021, 9(1): 1-27
25. Liu Q, Li L, Gao L, et al. Combination of bis (α‐furancarboxylato) oxovanadium (IV) and metformin improves hepatic steatosis through down‐regulating inflammatory pathways in high‐fat diet‐induced obese C57BL/6J mice[J]. Basic & Clinical Pharmacology & Toxicology, 2021, 128(6): 747-757.
26. Lei L, Huan Y, Liu Q, et al. Morus alba L.(Sangzhi) alkaloids promote insulin secretion, restore diabetic β-cell function by preventing dedifferentiation and apoptosis[J]. Frontiers in Pharmacology, 2022, 13.
27. Tong X, Gu J, Chen M, et al. p53 positively regulates osteoprotegerin-mediated inhibition of osteoclastogenesis by downregulating TSC2-induced autophagy in vitro[J]. Differentiation, 2020, 114: 58-66.
28. Zhang J, Jin W, Wang X, et al. A novel octreotide modified lipid vesicle improved the anticancer efficacy of doxorubicin in somatostatin receptor 2 positive tumor models[J]. Molecular pharmaceutics, 2010, 7(4): 1159-1168.
29. Li H, Li Y, Liang W, et al. The identification of PSEN1 p. Tyr159Ser mutation in a non-canonic early-onset Alzheimer's disease family[J]. Molecular and Cellular Neuroscience, 2022, 120: 103715.
30. Sun J, Xu S, Li H, et al. Galanin protects rat cortical astrocyte from oxidative stress: involvement of GalR2 and pERK1/2 signal pathway[J]. Mediators of Inflammation, 2019, 2019.
31. Wu X, Lin L, Wu H. Ferulic acid alleviates lipopolysaccharide‐induced acute lung injury through inhibiting TLR4/NF‐κB signaling pathway[J]. Journal of Biochemical and Molecular Toxicology, 2021, 35(3): e22664.
Zheng X, Zhang L, Wang W, et al. Anti-diabetic activity and potential mechanism of total flavonoids of Selaginella tamariscina (Beauv.) Spring in rats induced by high fat diet and low dose STZ[J]. Journal of Ethnopharmacology, 2011, 137(1): 662-668
相关产品
