CATALOG NUMBER: C1324

QUANTITY: 50µL

DESCRIPTION:

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein.

SPECIFICITY: The V5 tag antibody can recognize C-terminal, internal, and N-terminal V5-tag fusion proteins.

ISOTYPE: IgG1

TESTED APPLICATIONS: Western Blot,  Immunoprecipitation, Immunofluorescense

APPLICATIONS: WB: 1:5,000    IP: 1:200    IF: 1:1000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Immunoprecipitation:

IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate          2、transfected 293 cell lysate with V5-tag fusion protein

3、IP(untransfted 293+anti-V5 mAb+Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-V5 mAb+ Protein G agarose)

6、IP (transfected 293+Protein G)

7、Recombinant protein (E.coli)

Western Blot Immunofluorescense

1ug V5 fusion protein+ Primary antibody dilution IF analysis of 293 cells transfected with

at                                                      a V5 tag protein, using anti-

1、1:5,000                                          V5-Tag Mouse mAb at a 1:2000

2、1:10, 000 dilution (blue DAPI, red anti-V5)

For research use only; not for use as a diagnostic.

Important Note:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.

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