CATALOG NUMBER: C1343

QUANTITY: 50µL

DESCRIPTION:

The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding. They can also help to protect cells from stress. Hsp70 is usually in an ATP bound state. However it is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This interaction is reversible. Hsp70 in the ATP bound state may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.

SPECIFICITY: Human, Mouse, Rabbit

ISOTYPE: IgG1

MOLECULAR WEIGHT: ~70KD

TESTED APPLICATIONS: WB

APPLICATIONS: WB: 1:1,000-1:2000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

For research use only; not for use as a diagnostic.

Important Note:

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently dislodge any liquid in the container^,‘s cap.