CATALOG NUMBER: C1341

QUANTITY: 50µL

DESCRIPTION:

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

SPECIFICITY: Human, Rat, Mouse

ISOTYPE: IgG1

MOLECULAR WEIGHT: 29KDa

TESTED APPLICATIONS: Western Blot    Immunochemistry

APPLICATIONS: WB: 1:5,000    IHC: 1:200

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Western Blot

Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3) and 293T (4) with PCNA mouse mAb(12D10) diluted at 1:5000.

Immunochemistry

IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb(12D10) diluted at 1:200

For research use only; not for use as a diagnostic.

Important Note:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.