CATALOG NUMBER: C1907

QUANTITY: 50µL

BACKGROUND:

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.

SPECIFICITY: Antibody can detect endogenous Vimentin protein.

ISOTYPE: IgG1

APPLICATIONS: WB, IF, IHC

Species Cross-Reactivity: H, R, M

Recommended dilutions: WB: 1:1,000-2,000    IHC: 1:200-500

*NOTE: Optimal working dilutions must be determined by the end user.

STORAGE/HANDING: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Alternative Names: FLJ36605 antibody, VIM antibody, VIME antibody

Western Blot:

Western blot analysis of 1) Hela, 2)                Immunohistochemical analysis of paraffin-

Mouse Brain Tissue , 3) Rat Brain                 embedded human breast caricnoma using

tissue with TDY199 diluted at 1:2,000.             Vimentin （TDY199）Mouse mAb diluted at 1:500.

IF analysis of Hela with TDY199(Left) and DAPI (Right) diluted at 1:100.

For research use only; not for use as a diagnostic.

Important Note:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.