CATALOG NUMBER: C1810

QUANTITY: 100µL

DESCRIPTION:

The Akt proteins (Akt1, Akt2 and Akt3) are a family of cytoplasmic serine-threonine kinases that form a critical junction at the intersection of multiple cell signaling pathways. Also known as PKB, Akt is activated by multiple extracellular signals by phosphorylation at specific residues and is subsequently translocated to the plasma membrane. Akt is involved in the PI3K/AKT/mTOR pathway and other signaling pathways. The oncogenic properties of the Akt proteins are seen following mutation or constitutive activation in several different malignancies. Activity of the Akt proteins is negatively by PTEN, a protein tyrosine phosphatase tumor suppressor. Akt has also been shown to function as a survival factor in many cell types, an activity mediated by the phosphorylation of members of the bcl-2 family of proteins such as BAD.

SPECIFICITY: Human, Mouse, Rabbit

ANTIGEN: a 17 amino acid synthetic peptide from the C-terminus of human AKT1

CELLULAR LOCALIZATION: cytoplasm

MOLECULAR WEIGHT: ~60 kDa

APPLICATIONS: WB: 1:1,000-1:3,000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.

PRODUCT IMAGE:

Western blot analysis of cell extracts (40 ug of total protein was loaded) from NIH 3T3 fibroblasts.

For research use only; not for use as a diagnostic.

Important Note:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.